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KMID : 0385219940040010056
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Korean Journal of Gerontology
1994 Volume.4 No. 1 p.56 ~ p.60
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A Simple Electrochemical Method for the Production of Stable Superoxide Anion(O2-) in Acetonitrile Containing Tetrabutylammonium Bromide as the Electrolyte
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Ha Yeong-L.
Kim Jeong-O.
Csallany A. S.
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Abstract
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A rapid and simple electrochemical method for the production of stable superoxide anion(O2-)in acetonitrile containing tetrabutylammonium bromide(TBAB) as the electrolyte was developed. The production of O2- was performed at room temperature under the nitrogen atmosphere of the dry-glove box. The cathodic solution of H-type electrochemical cell separated by a sintered disc contained 30 ml of 0.1M TBAB solution, through which O2 was continuously bubbled. O2- was generated in the cathodic solution of the cell by the electrochemical reduction of molecular oxygen(O2) using the current(1, 2 and 5mA)and time(15, 20, 30 and 60min). Spectrophotometric property of the O2-was monitored, and appeared to be related to the electricity(mA x min) applied. The maximum absorbance(λmax)of the O2- generated with less than 40 electricity(1mA or 2mA for less than 20 min) was found to be 250nm. The λmax of O2- shifted to 252mm when generated with 60 electricity(2mA for 30 min), and reached at 260nm when generated with 120 electricity(2 mA for 30 min), and 50 reached at 260nm when generated with 120 electricity(2mA for 60 min). Absorbance at 250nm of the O2- produced with greater than 60 electricity(>2mA for 30 min) was slowly decreased by placing at room temperature for over a period of 20 min. The molar absorptivity of the O2- generated with 40 electricity(2mA x 20 min) was found to be 2.5 x 103m-1cm-1. These results suggest that the O2- generated in 0.1M TBAB/acetonitrile by the electrochemical reduction of O2 using 40 electricity(2 mA for 20 min) was the most stable at room temperature and could be useful for the study of its biological properties.
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KEYWORD
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superoxide anion(O2-), electrolyte, electrochemical reduction, molecular oxygen
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